Through hiring, training and guiding her team, she nurtures productive, long-lasting customer relationships. Her goal is to ensure that first-class service is provided by Artel’s customer-facing team, whether it’s directly interacting with customers or through her management of the team. Her background has given her hands-on knowledge of customers’ tests and assays, enabling her to understand their pain points since she has experienced them herself. Wendy puts her years of experience in the laboratory and her passion for helping people and problem solving to good use as Artel’s Technical Services Manager. Furthermore, this absorbance-based method provides the NIST traceability necessary for a standard method. Herein is presented the proof-of-concept for an approach which measures the accuracy of all dilution steps in a microplate using one multi-dye solution.

The limitations of both single dye absorbance and fluorescence methods require users to measure the accuracy of a subset of the total number of dilution steps forcing assumptions to be made about the repeatability of the liquid handler over multiple aspiration and dispense cycles. In contrast, fluorescent methods provide the required sensitivity, back lack the traceability pedigree associated with absorbance methods. In widely accepted single day absorbance QC methods, the dynamic range of the chomophore is too narrow to evaluate all of the dilution steps required to make the does response curve. Measurement of the dilution accuracy is not easily accomplished using traditional quality control methods. Absent reproducible volume control of the liquid handler the volume of any solution dispensed by it, and therefore the concentration of drug candidate, are questionable. Related to these assay steps, yet equally important to the success of the assay, is the ability of the liquid handler to accurately dispense the drug compound during the dose response analysis. There are many critical steps to this type of assay that are carefully controlled, such as contaminant quantitation and prospective drug concentration. The process of serially diluting solutions in microplates is common for many assays including preparation of dose response curves for IC50, drug efficacy testing, etc.

How to make serial dilutions